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Ana Sayago   Dr.  Other 
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Ana Sayago published an article in October 2018.
Research Keywords & Expertise
0 A
0 Olive Oil
0 Spectrophotometry
0 phenolic compounds
Top co-authors See all
Jose M. Vega

39 shared publications

Department of Plant Biochemistry and Molecular Biology, Faculty of Chemistry, University of Seville, 41012 Seville, Spain

Raúl González-Domínguez

39 shared publications

Department of Chemistry, Faculty of Experimental Sciences, University of Huelva, 21007 Huelva, Spain

Rosa María Martínez-Espinosa

38 shared publications

Biochemistry and Molecular Biology Division, Agrochemistry and Biochemistry Department, Faculty of Sciences, University of Alicante, E-03080 Alicante, Spain

Carlos Vílchez

20 shared publications

Algal Biotechnology Group, CIDERTA, RENSMA and Faculty of Sciences, University of Huelva, 21007 Huelva, Spain

Juan Urbano

19 shared publications

Laboratorio de Catálisis Homogénea; Unidad Asociada al CSIC; CIQSO-Centro de Investigación en Química Sostenible and Departamento de Química; Universidad de Huelva; Campus de El Carmen 21007 Huelva Spain

Publication Record
Distribution of Articles published per year 
(2002 - 2018)
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Publications See all
Article 0 Reads 0 Citations Optimization of Growth and Carotenoid Production by Haloferax mediterranei Using Response Surface Methodology Zaida Montero-Lobato, Adrián Ramos-Merchante, Juan Luis Fuen... Published: 09 October 2018
Marine Drugs, doi: 10.3390/md16100372
DOI See at publisher website ABS Show/hide abstract
Haloferax mediterranei produces C50 carotenoids that have strong antioxidant properties. The response surface methodology (RSM) tool helps to accurately analyze the most suitable conditions to maximize C50 carotenoids production by haloarchaea. The effects of temperature (15–50 °C), pH (4−10), and salinity (5–28% NaCl (w/v)) on the growth and carotenoid content of H. mediterranei were analyzed using the RSM approach. Growth was determined by measuring the turbidity at 600 nm. To determine the carotenoid content, harvested cells were lysed by freeze/thawing, then re-suspended in acetone and the total carotenoid content determined by measuring the absorbance at 494 nm. The analysis of carotenoids was performed by an HPLC system coupled with mass spectrometry. The results indicated the theoretical optimal conditions of 36.51 or 36.81 °C, pH of 8.20 or 8.96, and 15.01% or 12.03% (w/v) salinity for the growth of haloarchaea (OD600 = 12.5 ± 0.64) and production of total carotenoids (3.34 ± 0.29 mg/L), respectively. These conditions were validated experimentally for growth (OD600 = 13.72 ± 0.98) and carotenoid production (3.74 ± 0.20 mg/L). The carotenoid profile showed four isomers of bacterioruberin (89.13%). Our findings suggest that the RSM approach is highly useful for determining optimal conditions for large-scale production of bacterioruberin by haloarchaea.
Article 0 Reads 0 Citations High-Throughput Direct Mass Spectrometry-Based Metabolomics to Characterize Metabolite Fingerprints Associated with Alzh... Raúl González-Domínguez, Ana Sayago, Ángeles Fernández-Recam... Published: 18 September 2018
Metabolites, doi: 10.3390/metabo8030052
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Direct mass spectrometry-based metabolomics has been widely employed in recent years to characterize the metabolic alterations underlying Alzheimer’s disease development and progression. This high-throughput approach presents great potential for fast and simultaneous fingerprinting of a vast number of metabolites, which can be applied to multiple biological matrices including serum/plasma, urine, cerebrospinal fluid and tissues. In this review article, we present the main advantages and drawbacks of metabolomics based on direct mass spectrometry compared with conventional analytical techniques, and provide a comprehensive revision of the literature on the use of these tools in the investigation of Alzheimer’s disease.
Article 0 Reads 0 Citations Combination of complementary data mining methods for geographical characterization of extra virgin olive oils based on m... Ana Sayago, Raúl González-Domínguez, Rafael Beltrán, Ángeles... Published: 01 September 2018
Food Chemistry, doi: 10.1016/j.foodchem.2018.04.019
DOI See at publisher website
CONFERENCE-ARTICLE 6 Reads 0 Citations <strong>A short overview on the use of metabolomic multi-platforms in biomedical research</strong> Álvaro González-Domínguez, Ana Sayago, Ángeles Fernández-Rec... Published: 31 October 2017
MOL2NET 2017, International Conference on Multidisciplinary Sciences, 3rd edition, doi: 10.3390/mol2net-03-04637
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The integration of various analytical platforms is emerging as a very interesting alternative with the aim to maximize coverage in metabolomics. In this work, we present a concise review of the most commonly used metabolomic multi-platforms in biomedical research.

CONFERENCE-ARTICLE 5 Reads 0 Citations <strong>Development and Characterization of New Fermented Beverages of Low Alcoholic Graduation from Strawberry.</strong... Angeles Fernández Recamales, Ana Sayago, Raúl González-Domín... Published: 23 January 2017
MOL2NET 2016, International Conference on Multidisciplinary Sciences, 2nd edition, doi: 10.3390/mol2net-02-03892
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Nowadays, the oenological market tends to the diversification through the development of new original and enriched products. Thus, the  study here proposed pretends the elaboration and characterization of new "wine-type" beverages based on the use of berries, resulting in products with added value due to the increase in their content of bioactive compounds.

Strawberry is a fruit with a great economic relevance in Spain, being the first producer and exporter country in Europe. On the other hand, a large amounts of surplus from this fruit are obtained and destined to industrial purposes every year. Taking into account this, the main objective of this work has been to study the possibility of providing another utility to these surpluses, as well as to diversify the wine production towards new elaborations that could be adapted to the markets. Therefore, the transformation of this surplus from strawberry  in more durable new products via fermentation could be an economically viable solution for the industry and producers.

These new products would allow tasting the fragrance and exquisiteness of strawberries in form of a beverage elaborated on the basis of traditional procedures for winemaking. For this, a puree of strawberry of 7 º Brix was mixed with water and sugar to reach 12 º Brix in order to obtain a fermented product with an alcoholic graduation between 6-7 º. Periodic controls of density, temperature, pH, acidity, and ºBrix were performed during the vinification process. At the end of this process, potassium metabisulfite was added to stabilize the product.

The "strawberry wine" obtained was characterized by the determination of parameters related to organoleptic, nutritional and nutraceutical quality (phenolic and volatile profile, acids and sugars, antioxidant capacity and color parameters). The results obtained were compared with those of samples of commercial fruit wines (elderberry, blackcurrant and cherry).

MOL2NET 2016, International Conference on Multidisciplinary Sciences, 2nd edition, doi: 10.3390/mol2net-02-03880
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In recent years, interest in phenolic compounds has been increased due mainly to the numerous evidences of its beneficial health effects and their impact on food quality. Numerous efforts have been made in order to increase the knowledge about phenolic compounds, with an especial focus on characterization of new food sources rich in polyphenols. There has been studied the influence of different factors such as variety, pedoclimatic conditions, geographical origin, authenticity and traceability, among others on their contents, as a tool for enhancing nutritional and nutraceutical quality of plant derived foods in breeding programs.
The objective of the present study was to assess whether growth under controlled condition could be used for cultivation of strawberry to enhance bioactive polyphenols content. For this purpose, 54 samples of strawberries belonging to three varieties with different sensitivity to environmental conditions (Camarosa, Festival, Palomar) were grown in soilless system with different agronomic conditions (electrical conductivity, substrate type and coverage).
UHPLC-ESI-MS / MS analysis of polyphenolic compounds combined with chemometric methods revealed that benzoic acids, cinnamic acids, flavan-3-ol and antioxidant activity were significantly influenced (p <0.001) by coverage and variety. In addition, it was observed that the substrate has a significant effect on flavonols, whereas the variety affects the content of ellagic acid and its derivatives as well as in anthocyanins.